mouse monoclonal anti nucleolin  (Santa Cruz Biotechnology)

Journal: Frontiers in Microbiology

Article Title: Anaplasma phagocytophilum AFAP targets the host nucleolus and inhibits induced apoptosis

doi: 10.3389/fmicb.2024.1533640

Figure Lengend Snippet: Top 25 most abundant AFAP-interacting proteins.

Article Snippet: Permeabilized cells were incubated with a mouse monoclonal anti-nucleolin antibody (cat#:sc-17826, Santa Cruz Biotechnology) or mouse monoclonal anti-NPM1 antibody (cat#: sc-32256, Santa Cruz Biotechnology) at the dilution of 1:100 for 1 h at 37°C before incubation with Alexa Fluor 555-conjugated goat anti-mouse IgG antibody (1:200 dilution, Molecular Probes, Invitrogen).

Techniques:

Journal: Frontiers in Microbiology

Article Title: Anaplasma phagocytophilum AFAP targets the host nucleolus and inhibits induced apoptosis

doi: 10.3389/fmicb.2024.1533640

Figure Lengend Snippet: Colocalization assay for AFAP and nucleolin, and AFAP and NPM1. RF/6A cells were transfected for 1 day with a plasmid expressing AFAP-GFP (A,B) or GFP (C,D) , followed by incubation with mouse monoclonal anti-nucleolin (A,C) or anti-nucleophosmin (B,D) antibody and Alexa Fluor 555-conjugated goat anti-mouse IgG antibody. DAPI was used to stain the nucleus. Scale bars: 10 μm.

Article Snippet: Permeabilized cells were incubated with a mouse monoclonal anti-nucleolin antibody (cat#:sc-17826, Santa Cruz Biotechnology) or mouse monoclonal anti-NPM1 antibody (cat#: sc-32256, Santa Cruz Biotechnology) at the dilution of 1:100 for 1 h at 37°C before incubation with Alexa Fluor 555-conjugated goat anti-mouse IgG antibody (1:200 dilution, Molecular Probes, Invitrogen).

Techniques: Transfection, Plasmid Preparation, Expressing, Incubation, Staining

Journal: Scientific reports

Article Title: mRNA in situ hybridization exhibits unbalanced nuclear/cytoplasmic dystrophin transcript repartition in Duchenne myogenic cells and skeletal muscle biopsies.

doi: 10.1038/s41598-023-43134-6

Figure Lengend Snippet: Figure 10. Dual RNAscope and immunostaining in WT1 and DMD immortalized myoblasts. (A) 37–42 and 63–75 RNAscope probes were used in combination with a monoclonal antibody against the human nucleolin protein. Each single DMD transcript is represented as a distinct red dot, whereas nucleolin protein is represented as a green signal. Counterstain: Gill’s Hematoxylin. (B) Colocalization of dystrophin transcript and nucleolin protein in immortalized myoblasts. Using the 37–42 probe, we found about 45% in WT1 and a mean of 42% in DMD of Dp427 overlapping with nucleolin signals. No statistically significant differences were observed between samples. Using probe 63–75, we showed lower dystrophin and nucleolin colocalization in both WT1 (26%) and DMD (mean of 34%). No statistically significant differences were found for 63–75 signal between WT1 and DMD. Bar: SEM. **p < 0.001; *p < 0.05.

Article Snippet: Nucleolin protein was immunoprecipitated with anti-nucleolin C23 MS-3 mouse monoclonal antibody (5 μg; Santa Cruz) using the Magna RIP RNA-Binding Protein Immunoprecipitation Kit (Millipore).

Techniques: RNAscope, Immunostaining